Immunoneutralization of Growth Differentiation Factor 9 Reveals It Partially Accounts for Mouse Oocyte Mitogenic Activity1

Oocyte-specific expression of growth/differentiation factor-9.

Growth/differentiation factor-9 (GDF-9) is a previously described member of the transforming growth factor-beta superfamily expressed specifically in the ovary in adult mice. Using in situ hybridization methods, we have localized the expression of GDF-9 messenger RNA (mRNA) exclusively to oocytes. GDF-9 mRNA was detected in oocytes at all stages of follicular development, except in primordial f...

Screening for FecGH Mutation of Growth Differentiation Factor 9 Gene in Iranian Ghezel Sheep Population

Background Ghezel sheep are highly prolific and one of the local sheep breeds in Iran and Turkey. Growth differentiation factor-9 (GDF9) gene has been found to be essential for growth and differentiation of early ovarian follicles. Novel mutations in GDF9 have been associated with increased ovulation rates and high litter sizes in heterozygous carriers. Therefore, fecundity gene for GDF9 (FecGH...

Simultaneous Presence of G1 and G4 Mutations in Growth Differentiation Factor 9 Gene of Iranian Sheep

The fecundity in sheep has been much interested for producers due to its economical importance. Growth differentiation factor 9 (GDF9) is an autosomal gene with over dominance effect for twosingle nucleotide polymorphisms(SNPs), which resulted in sterility in homozygote mutant ewes and additive effect for three SNPs, without showing sterility event. In the present study, DNA was extracted from ...

I-9: Proteomic Analysis of Mouse Oocytes Reveals 28 Candidate Factors of the

P-128: Fibroblast Growth Factor Improves Oocyte Maturation in Vitrified-Thawed Mouse Follicles

Background: The fibroblast growth factors (FGF-4) are a group of heparin-binding single chain polypeptides that play a pivotal role in development, cell growth, tissue repairing and transformation. The aim of this study is to improve development and maturation outcome of vitrified mouse pre-antral follicle by adding of fibroblast growth factor (FGF) into medium culture. Materials and Methods: P...